Each substrate is sold separately:

PRINCIPLE/DISCUSSION:

The alkalinization of amides and organic salts can often be used as a tool to identify non-fermentative bacilli when other tests are inconclusive. Key Scientific offers tablets for 5 amides and 2 salts.

ACTIVE INGREDIENTS:

Each tablet contains 2 mg. of the respective salt or amide except the Niacinamide tablet which contains 5 mg.

MATERIAL SAFETY DATA:

This product does not contain any materials known at this time to be hazardous.

STORAGE:

Store tightly covered and with desiccant in a dry place at room temperature.

MATERIALS REQUIRED:

Amide and Organic Salt Tablets require fresh 18-24 hour growth on TSI or KIA slants. The following items are required but not provided: Stoppered test tubes, Boiling water bath, Sterile distilled water, pipette or inoculating loop

PROCEDURE:

(1)Place one tablet and 1 ml of distilled water in a stoppered or capped tube.

(2)Boil in a boiling water bath for 10 minutes to kill any vegetative cells which might be present.

(3)Cool. With a sterile pipette, inoculate with 2 drops of a heavy bacterial suspension made by mixing the growth from a TSI or KIA slant in 1.5 - 2.0 ml. of sterile distilled water. This should be sufficient to inoculate all 7 tubes. If you are only testing with one or two substrates, you may simply inoculate the media with a loopful of growth from the slant.

(4)Incubate at 37C for 4-6 days, observing daily for a color change.

INTERPRETATION:

A color change from yellow to purple is a positive result, indicating that the amide or salt has been alkalinized.

QUALITY CONTROL:

Tablets should be tested with known positive and negative organisms. Dispose of used materials in a manner appropriate for biohazardous materials.

SOME EXPECTED REACTIONS: