K375 LEAD ACETATE STRIPS

PRINCIPLE/DISCUSSION:

Key Lead Acetate Strips are used to detect the production of hydrogen sulfide by Enterobacteraciae. The strips are more sensitive than TSI or KI agar and may be positive when the agar butt reaction would be inconclusive.

ACTIVE INGREDIENT:

Each strip is impregnated with a solution of lead acetate and water.

MATERIAL SAFETY DATA:

Lead is harmful if ingested in large quantities. If this occurs, contact a physician.

STORAGE:

Store tightly covered in a dry place at room temperature.

QUALITY CONTROL:

Strips should be tested before use with known hydrogen sulfide positive and negative organisms. Dispose of all used material in a manner appropriate for biohazardous material.

MATERIALS REQUIRED BUT NOT PROVIDED:

Lead Acetate Strips require fresh 24 hour growth on media appropriate for the specimen. Consult a clinical microbiology manual for suggestions. The following items are required but not provided: Culture media containing sulphur (ex. KIA or TSIA) Inoculating loop

MATERIALS PROVIDED:

LEAD ACETATE STRIPS-50 PER BOTTLE

PROCEDURE:

(1)Inoculate a tube of liquid media containing sulphur compounds.

(2)Insert a Key Lead Acetate Strip between the plug and inner wall of the tube, above the level of the media.

(3)Follow the directions of the media you are using for incubation length and temperature.

(4)After incubation observe for blackening of the strip.

INTERPRETATION OF RESULTS:

The blackening of the lead acetate strip indicates production of hydrogen sulfide gas in the tube.

SOME EXPECTED REACTIONS:

Positive: Proteus, Salmonella (most serotypes),Citrobacter freundii

Negative: Citrobacter diversus, E. coli, Shigella species, Klebsiella, Enterobacter, Providencia

REFERENCES:

(1) Bailey and Scott's Diagnostic Microbiology, Fifth Edition, Chapter 43 "Reagents and Tests".

92258J