PHENYLALANINE DEAMINASE TABLETS

K1530 - WEE-TAB 28 bottle, K530 - 50 per vial

DISCUSSION:

A few organisms produce the enzyme which deaminates phenylalanine to produce phenylpyruvic acid. The most familiar of these are the Proteus and Providencia species, Morganella morganii, and Enterobacter agglomerans. Key Phenylalanine Deaminase Tablets provide the substrate necessary to detect the enzyme. The phenylpyruvic acid end product reacts with ferric chloride to produce a green color.

ACTIVE INGREDIENT:

Each tablet contains phenylalanine hydrochloride.

MATERIAL SAFETY DATA:

This product does not contain any material known at this time to be hazardous.

INSTRUCTIONS:

Regular tablets:

1) In a small test tube, dissolve 1 tablet in 1 ml. of distilled water.

2) Inoculate heavily. One loopful of growth from a culture plate should be enough.

3) Incubate at 37 degrees for 24 hours.

(4) Add 1-2 drops of ferric chloride reagent. A green color appearing within 1-5 minutes is a positive result.

WEE-TABS:

1) Add 5 drops of water to the tube containing the tablet. If using bulk tablets, add 1 tablet to a small test tube before adding water.

2) Inoculate heavily with a loopful of growth, mixing well.

3) Incubate for 4-6 hours.

4) Add 1 drop of ferric chloride and observe for development of green color.

MATERIALS REQUIRED:

Phenylalanine tablets are sold in quantities as listed above. All tests require fresh 24 hour growth on media not available from Key. Consult the Manual of Clinical Microbiology for suggested media. Other materials required but not provided: Ferric chloride solution (available from Key Scientific in 3 ml. and 25 ml. bottles), loop for harvesting colonies, and distilled water

STORAGE:

Store tightly covered in at room temperature.

QUALITY CONTROL:

Phenylalanine deaminase tablets should be tested before use with organisms of known reactivity. Discard used tests in a manner conforming with accepted laboratory procedures for biohazardous materials.

REFERENCES AND FOOTNOTES:

(1) Bailey and Scott's Diagnostic Microbiology, 8th Edition, Chapter 27.

(2) Manual of Clinical Microbiology, 5th Edition, Chapter 36.

SOME EXPECTED REACTIONS:

Citrobacter -

Enterobacter -

E. coli -

Klebsiella -

Shigella -

Salmonella -

Morganella +

Proteus +

Providencia +

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